Figure 3.

Analysis of hTERT expression, proliferation rates and senescence marker β-galactosidase of hTERT-transduced ADSC-derived sub-populations. (A) Expression of hTERT in 18 hTERT-transduced ADSC sub-populations as revealed by Western Blot technique. (B) Comparison of proliferation rates of hTERT ADSC-derived sub-populations. Note that two subpopulations (S1 and S8-ADSC) had high proliferation activity, while the rest of the tested cells stopped proliferating after 29–30 population doublings. (C) Senescence-associated β-galactosidase assay performed at passage #10 after transduction, showing the lack of enzyme activity in high-proliferative sub-populations S1 and S8-ADSC and a noticeable activity in sub-population S5-ADSC, which stopped proliferating after 30 population doublings (β-galactosidase positive cells are indicated with white arrows).