Table 2.
Antibodies and Other Reagents | Source | Dilution | Label |
---|---|---|---|
Goat anti-mouse IgG Ab (#ab97035) | AbCam, United Kingdom | 1/500 | Cy3 |
Goat anti-rabbit IgG Ab (#ab150077): | AbCam, United Kingdom | 1/500 | Alexa Fluor 488 |
AmpliStain™ anti-Mouse 1-Step HRP (#AS-M1-HRP) | SDT GmbH, Baesweiler, Germany |
ready-to-use | HRP |
AmpliStain™ anti-Rabbit 1-Step HRP (#AS-R1-HRP) | SDT GmbH, Baesweiler, Germany |
ready-to-use | HRP |
4’,6-diamidino-2-phenylindole (DAPI, #D9542-5MG) | Sigma, Hamburg, Germany |
5 µg/mL | w/o |
VECTASHIELD®® Mounting Medium (#H-1000) | Vector Laboratories, Burlingame, CA, USA | ready-to-use | w/o |
DAB Peroxidase Substrat Kit (#SK-4100) |
Vector Laboratories, Burlingame, CA, USA | ready-to-use | DAB |
Mayer’s hematoxylin (#MHS128) | Sigma-Aldrich | ready-to-use | w/o |