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. 2022 Aug 10;23(16):8926. doi: 10.3390/ijms23168926

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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METH dose-dependently reduces MTR-R fluorescence. MTR-R labels healthy mitochondria. (A) Representative pictures showing a dose-dependent decrease of MTR-R fluorescence for doses starting at METH 100 μM. Arrows indicate intensely MTR-R-stained cells. (B) The graph reports the mean densitometry of MTR-R fluorescence per cell. Values are given as the mean percentage ± S.E.M. of optical density (assuming controls as 100%) from three independent experiments. * p < 0.05 compared with controls. Scale bar = 11 μm.