TABLE 3.
Lithoautotrophic growth, hydrogenase activitie, and activities of the SH and MBH promoters in wild-type and mutant strains of A. eutrophus
| Strain | Growth on H2a | Activity (U)b
|
|||
|---|---|---|---|---|---|
| Hydrogenase
|
β-Galactosidase
|
||||
| SH | MBH | PSH (pGE320) | PMBH (pGE319) | ||
| H16 | + | 2.1 ± 0.1 | 0.6 ± 0.1 | 12,961 ± 960 | 14,445 ± 773 |
| HF457 (H16 hoxTΩR-B2) | − | 0.3 ± 0 | 0.0 | 1,115 ± 32 | 942 ± 32 |
| HF491 (H16 hoxKΔ171-R4) | − | ND | ND | 4,039 ± 48 | 3,033 ± 158 |
a
Single colonies were picked and suspended in phosphate buffer. The optical density of the suspension was normalized, and the same volume of each was streaked on minimal salts plates. The plates were incubated for 3 days under a standard gas mixture consisting of 80% H2, 10% CO2, and 10% O2.
b
FGN cultures; mean values ± standard errors; n = 5. ND, not determined.