Table 4.
Primers used in this study. The Nde I and Sac I sites used for vector construction are underlined.
| Assay | Primer Name | Sequence (5′–3′) |
|---|---|---|
| Gene expression | MdAux/IAA2-ex-F | GAACTTGTTGTGGTGTGGAG |
| MdAux/IAA2-ex-R | CGCTGATTGCTACTAAGCTG | |
| MdActin-F | GGCTGGATTTGCTGGTGATG | |
| MdActin-R | TGCTCACTATGCCGTGCTCA | |
| Gene cloning | MdAux/IAA2-full-F | ATGGATAATTTGTATAGTATGAT |
| MdAux/IAA2-full-R | TCATCTTCCAATTCCAACTT | |
| MdAux/IAA2-pro-F | AGATAGCTAAGAATTTCTGGCGTA | |
| MdAux/IAA2-pro-R | GCCCTTACAACTCCATTATTCTCA | |
| Vector construction | pRI101-MdAux/IAA2-OE-F | TCTTCACTGTTGATACATATGATGGATAATTTGTATAGTATGAT |
| pRI101-MdAux/IAA2-OE-R | CGATCGGGGAAATTCGAGCTCTCATCTTCCAATTCCAACTT | |
| pRI101-MdAux/IAA2-AN-F | CGATCGGGGAAATTCGAGCTCATGGACGGGAAGAAACTGGA | |
| pRI101-MdAux/IAA2-AN-R | TCTTCACTGTTGATACATATGCTAAGGGATCCACTTAGGATCT |