Figure 2.

The inhibitory effect of HVE (a) and HT (b) treatment (24 h) on the release of TSLP using differentiated HaCaT cells challenged by TNF-α (10 ng/mL)/IL-4 (100 ng/mL), measured by the ELISA assay. The highest concentration of HT (10 μM) corresponds to 4.93 μg/mL. The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. * p < 0.05, ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; HT, hamamelitannin; reference inhibitors: A, apigenin (20 μM); S6I, STAT-6 inhibitor (50 nM).