Figure 5.
The inhibitory effect of HVE (a) and HT (b) treatment (24 h) on the release of CCL26 using differentiated HaCaT cells challenged by IL-4 (100 ng/mL), measured by the ELISA assay. The effect of HT (10 μM corresponding to 4.93 μg/mL) on HVE IC50 (20 μg/mL) is also reported (c). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; HT, hamamelitannin; reference inhibitors: A, apigenin (20 μM); S6I, STAT-6 inhibitor (50 nM).