FIG. 5.

Map of in-frame fusion construct cti::phoA in plasmid pJBctiphoA and growth-dependent alkaline phosphatase activity in wild-type P. putida DOT-T1E transformed with plasmid pJBctiphoA. (A) The cti::phoA fusion in pJB3Tc19 yielded pJBctiphoA as described in the text. DNA from strain DOT-T1E is shown as a hatched area, and vector pJB3Tc19 is shown as thick lines. (B) PhoA activity of the cti::phoA fusion was measured in the presence and absence of toluene at the indicated times. ■ and □, DOT-T1E with pJBctiphoA grown on glucose; ▴ and ▵, DOT-T1E with pJBctiphoA grown on toluene in the gas phase; ● and ○ DOT-T1E with pJBctiphoA grown on glucose and toluene in gas phase. Solid symbols indicate growth curve, and open symbols indicate phosphatase activity (micromoles of p-nitrophenol per minute per turbidity unit [measured as optical density, OD, at 660 nm]).