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. 2022 Aug 21;23(16):9439. doi: 10.3390/ijms23169439

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Change trends of physiological parameters of transgenic lines and WT plants under freezing treatment. The chlorophyll fluorescence image (a) and Fv/Fm values (b) of PgCBF3 and PgCBF7 transgenic Arabidopsis under freezing stress; (c) H₂O₂ accumulation by DAB chemical staining; (d) detection of O^2- by NBT chemical staining electrolyte leakage (e) and MDA (f) content of PgCBF3 and PgCBF7 transgenic Arabidopsis at freezing temperature. Different letters present significant differences (p < 0.05). CA: cold-acclimated; NA: non-acclimated.