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. 2022 Aug 15;23(16):9134. doi: 10.3390/ijms23169134

Figure 5.

Figure 5

Metformin decreased LLC cell proliferation. Metformin (0–10 mM) was added to the cultured media of LLC cells for 24 h. Cell viability was measured using a CCK-8 kit (A) and caspase 3 activity was measured using an enzymatic assay kit (B). Metformin (0–30 mM) was added to the cultured media of LLC cells for 6 days. Cell colonies were visualized using crystal violet staining. Representative images and quantitative data are shown (C). Metformin (0–1 mM) was added to the cultured media of LLC cells, and cell migration within a course of 24 h was measured using a Transwell-based method. Representative photomicrograph and representative data are shown (D). * p < 0.05 vs. metformin (0 mM), n = 4.