Figure 8.

Metformin mitigated tissue parameters of NETs in obese mice. ND-fed lean and HFD-fed obese mice were subcutaneously inoculated with LLC cells over a course of three weeks. Three days after cell implantation, ND-fed lean mice were daily treated with saline vehicle through intraperitoneal injection (ND). HFD-fed obese mice were separated into two groups receiving daily intraperitoneal injections of a saline vehicle (HFD) or metformin (50 mg/kg, HFD/Metformin) for the final 18 days. Immunohistochemical examination was performed in paraffin-embedded tumor tissues with indicated antibodies. Representative photomicrograph and quantitative data are shown, as described in methodology. * p < 0.05 vs. ND and # p < 0.05 vs. HFD, n = 6. Scale bar: 50 μm.