Table 2.
Ability of E. coli strains to survive within RAW 264.7 macrophages *.
| Strains | Initial Uptake (3 h.p.i) | % 24 h.p.i | ||
|---|---|---|---|---|
| 1I06 | 292.0 | ±78.0 | 6.9 | ±0.5 |
| 2C05 | 143.0 | ±11.9 | 3.3 | ±1.0 |
| 4C01 | 679.8 | ±108.5 | 409.8 | ±71.7 |
| 4I01 | 606.8 | ±104.5 | 127.3 | ±21.1 |
| 5C08 | 124.3 | ±15.2 | 16.3 | ±4.4 |
| 5I01 | 168.7 | ±10.5 | 83.8 | ±16.6 |
| 6I01 | 45.1 | ±39.1 | 5.1 | ±4.1 |
| 6I09 | 110.7 | ±9.9 | 27.0 | ±10.6 |
| 6I10 | 8.4 | ±1.0 | 1.0 | ±0.3 |
| 7C02 | 737.0 | ±148.6 | 5.7 | ±1.3 |
| 7C08 | 128.7 | ±23.8 | 3.0 | ±1.9 |
| 9C01 | 250.7 | ±44.1 | 59.8 | ±12.4 |
| 10C01 | 50.6 | ±4.6 | 9.0 | ±3.9 |
| 10I01 | 88.7 | ±6.2 | 30.0 | ±7.0 |
| 18I08 | 83.2 | ±16.8 | 45.1 | ±7.7 |
| 24C01 | 119.2 | ±9.5 | 0.003 | ±0.004 |
| NRG 857c | 74.3 | ±5.2 | 31.3 | ±5.2 |
| HM605 | 58.3 | ±9.7 | 22.9 | ±4.4 |
| HB101 | 29.7 | ±7.1 | 0.0 | ±0.0 |
* The table shows the initial uptake, defined as the mean CFUs resisting amikacin treatment after 3 h.p.i, and the percentage of bacterial content at 24 h.p.i compared to the initial uptake. The HB101 strain was used as a negative control. Those strains that survived within macrophages according to the criteria used as reference (equal to or greater than the values exhibited by the AIEC NRG857c and HM605 strains) are highlighted in gray. Mean and standard deviation indicated correspond to three independent experiments performed in duplicate.