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. 2022 Aug 14;23(16):9108. doi: 10.3390/ijms23169108

Figure 1.

Figure 1

Hepatocyte growth factor (HGF) and epidermal growth factor (EGF) suppress proliferative activity in HepG2 cells treated with 5-fluorouracil (5-FU). HepG2 cells were cultured for 24 h in serum-starved medium. Incubation was continued with addition of either HGF (50 ng/mL) or EGF (50 ng/mL) for further 7 h. Thereafter, 5-FU (25 µg/mL: (A) 50 µg/mL: (B) 75 µg/mL: (C) 100 µg/mL: (D) 125 µg/mL: (E)) and vehicle (F) were added. The proliferative activity was measured at the indicated times and is expressed as a percentage of the proliferative activity of cells not treated with growth factor and 5-FU at 0 h (A–E) or at the corresponding times (F). Each bar represents the mean ± SD of 5 experiments.