Figure 1.

Panel (A) shows the position of molecular mass markers. The IgG_mix (14 μg) homogeneity analysis by SDS-PAGE under non-reducing conditions in the absence of thiol-disulfide reducing reagent dithiothreitol (B); silver staining. Panel (B) demonstrates the position of IgGs. The relative activities (RA, %) in the hydrolysis of five histones (■) and MBP (□) were estimated using eluates of gel fragments (2–3 mm) (C). After incubation for 24 h with eluates, complete hydrolysis of all substrates was undertaken for 100% (C). The errors of the relative activities estimation from two independent experiments did not exceed 7–10%.