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MALDI spectra corresponding to IgGs (0.045 mg/mL) incubated in the absence of H4 histone (A), products of H4 histone (0.8 mg/mL) hydrolysis in the absence (B) and the presence of IgGs ((C,D) 0.045 mg/mL) against five histones before immunization with antigens ((C) zero time—Con-0d-His preparation) and after spontaneous development of EAE during 60 days (Con-60d-His preparation) (D).
