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. 2022 Aug 18;23(16):9309. doi: 10.3390/ijms23169309

Figure 2.

Figure 2

Lipid peroxidation-related and antioxidative parameters in liver tissue in control (Ctrl), diabetic (DM), and diabetic Fer-1-treated (DM + Fer-1) animals. (a) DAB-enhanced Pearls’ iron staining demonstration of ferrous ions accumulation (arrows); Sudan III stain-detected lipofuscin (arrows) and immunohistochemical detection of 4-HNE; scale bars: iron and 4-HNE—50 µm, lipofuscin—20 µm. (b) Immunohistochemical detection of GPX4; scale bars: 50 µm and (c) quantification of tissue and nuclear GPX4 immunopositivity; cv—centrilobular vein, pv—portal vein. (d) Total SOD activity. (e) Protein content of pACC; β-actin serves as a protein-loading control; blots represent three independent experiments. Values presented as means ± SEM. Statistical significance: compared with the Ctrl group (*), * p < 0.05, *** p < 0.001; DM vs. DM + Fer-1 comparison (#), ## p < 0.01; ### p < 0.001.