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. 2022 Aug 22;23(16):9499. doi: 10.3390/ijms23169499

Figure 4.

Figure 4

Fission yeast Cka1 and Ckb1 can modulate basal transcription in a fission yeast whole cell extract. (A) Basal transcription (lane 1), labeled (+) in the presence of Cka1 (2.0 and 4.0 pmol, lanes 3 and 4, respectively), can downregulate basal transcription from the Ad-MLP promoter. Ckb1 (2.0 and 4.0 pmol, lanes 5 and 6) added to the transcription assay has no effect on transcription. CKa1 (4 pmol) in the presence of Ckb1 (1.0, 2.0 and 4.0 pmol, lanes 7–9l) restores basal transcription. The lane 2 labeled (−) represents the negative control without DNA template. (B) The signals of each lane from (A) were quantified and plotted using the Image J software. (C) Lane 1 (+) represents basal transcription and the lane labeled TFIIEβ represents the basal transcription with this polypeptide added, while the lane labeled PC4 represents basal transcription in which PC4 was added. Cka1 was added to the basal assay in the lane 5 labeled Cka1. Lane 6 labeled +TFIIEβ represents an assay in which Cka1 and TFIIEβ were added simultaneously. Fission yeast PC4 (40, 80 and 160 ng, lanes 7–9) was added in those lanes labeled PC4, in the presence of 4 pmol of Cka1. Lane 2 labeled (−) represents a negative control without template. (D) Those signals of each lane from (C) were quantified and plotted using the image J software; ns indicates no significative, **** indicates p < 0.0001.