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. 2022 Aug 11;23(16):8960. doi: 10.3390/ijms23168960

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Targeted LC/MS/MS extracted ion chromatograms for light and heavy versions of GFAP peptides in control and in acute and subacute time points: (A) DGEVIKES and (B) DGEVIKE. Heavy peptides were spiked in at 20 fmol/50 uL of pooled CSF before ultrafiltration with a 10 kDa membrane. Pooled CSF samples were generated by pooling 10 uL of each sample type (control, n = 14; acute TBI, n = 15; subacute TBI, n = 13) from the available CSF samples. The data shown are for a single analysis of each pooled sample type.