. 1999 Sep;181(18):5725–5733. doi: 10.1128/jb.181.18.5725-5733.1999

TABLE 1.

Kinetic parameters for purified wild-type, recombinant, and mutant Qdo enzymes

Enzyme	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ μM⁻¹)
Qdo (wild-type)	24^a	ND^b
Qdo (recombinant, His₆ tagged)	20	2,214	110.70
QdoS95C (His₆ tagged)	61	7	0.11
QdoS95A (His₆ tagged)	95	164	1.73
QdoH244A (His₆ tagged)	BD^c	BD	BD
QdoD120A (His₆ tagged)	35	1,298	37.09
QdoD219A (His₆ tagged)	50	397	7.94

Data from reference 7.

ND, not determined.

BD, activity was below the limit of detection.