Skip to main content
. 2022 Aug 20;23(16):9420. doi: 10.3390/ijms23169420

Figure 4.

Figure 4

Bbs10 depletion affects energy metabolism in kidney-derived epithelial cells. (a) Relative mRNA expressions levels of key metabolic enzymes including Pdk1, Ldh1, Pkm2, Hk-1, Cpt-1, Cpt2, Acox-1, and Glut-1 (H) in IMCD3-Bbs10-/-cells (n = 20) and wild type cells (n = 5). Data are presented as mean ± SEM and are normalized to wild type controls as the y-axis is indicated by the fold. The statistical significance of the difference in mRNA expressions between conditions (IMCD3-Bbs10-/- cells and wild type controls) was evaluated by an unpaired parametric t-test with Welch correction (* p < 0.05, ** p < 0.01, **** p < 0.0001, ns = not significant). (b) Western blot analysis of the expression of hexokinase and Glut-1 in IMCD3-Bbs10-/- cells and wild type. β-actin was used as the loading control. (c) Quantitative densitometric analysis was performed for data analysis and showed an increase in Glu-1 and Hk-1 also at protein levels (p-value < 0.05). Data are shown as the means ± SEM and are representative of three independent experiments, each performed in triplicate.