Figure 4.

Oxidative phosphorylation (OX-PHOS) of kidney mitochondria after 20 min of bilateral renal ischemia where the mice body temperature was maintained at normothermia (IR-37 °C) or at mild therapeutic hypothermia (mTH) (IR-34 °C), and 2 h (a,c,e) or 24 h (b,d,f) of reperfusion, or after a Sham procedure with the same body temperature (Sham-37 °C and Sham-34 °C). Sensitive rates of OX-PHOS of electron transport chain (ETC) complex I, II, and IV were measured through O₂ consumption of 250 µg kidney mitochondria. For each complex, state 3 represents the maximal oxygen consumption stimulated by adenosine diphosphate (ADP) when the substrates of the different complexes are present. OX-PHOS of complexes I, II, and IV were altered as soon as 2 h after reperfusion, and only OX-PHOS of complex II and IV remained altered after 24 h of reperfusion, compared to Sham. mTH prevented those alterations at 2 h and 24 h of reperfusion. Data are shown as median with interquartiles. * p < 0.05, Mann–Whitney test. + p < 0.05, ++ p < 0.01 vs. Sham-37 °C, Mann–Whitney test.