Figure 2.
Effect of crude C. majus latex, protein fraction, and alkaloid-rich fraction on HaCaT cell viability. Consecutive numbers (1–7) on the x axis correspond to different protein concentrations in the crude latex (S1) and protein fraction (S2) applied on cells: 1 = 4.0 × 10−4 mg/mL, 2 = 8.0 × 10−4 mg/mL, 3 = 1.6 × 10−3 mg/mL, 4 = 8.0 × 10−3 mg/mL, 5 = 1.6 × 10−2 mg/mL, 6 = 4.0 × 10−2 mg/mL, 7 = 8.0 × 10−2 mg/mL. In the case of alkaloid fraction (S3), consecutive numbers (1–7) correspond to its different dilutions: 1 = 5 × 10−4, 2 = 10−3, 3 = 2 × 10−3, 4 = 10−2, 5 = 2 × 10−2, 6 = 5 × 10−2, 7 = 10−1. They were obtained from series of dilutions from concentrated samples. Among the tested samples, the crude latex and the alkaloid-rich fraction showed the strongest cytotoxicity in the range of 8.0 × 10−3–8.0 × 10−2 mg/mL (numbers 4–7), and the protein fraction exerted lowest toxicity against HaCaT cells in this concentration range. Most of the tested samples in the range of 4.0 × 10−4–1.6 × 10−3 mg/mL (numbers 1–3) showed the lowest cytotoxicity.