Figure 4.

Effect of C. majus latex (S1), protein fraction (S2), and alkaloid-rich fraction (S3) on the attachment of HPV pseudovirions to the surface of HaCaT cells. HPV pseudoviral particles were applied to HaCaT cells that had previously been incubated with crude C. majus latex, alkaloid-rich fraction, or protein fraction. The amount of pseudoviral particles bound to the cells was then determined by Western blot and L1-specific antibody. (A) Representative Western blot result after immunodetection. S1—treatment with crude latex, S2—treatment with protein fraction, S3—treatment with alkaloid-rich fraction, C⁻—sample without PsV-HPVs, C⁺—untreated cells. (A) Clear signal for all tested samples indicates the lack of influence of the crude C. majus latex, alkaloid-rich fraction, and protein fraction on the stability of the PsV-HPV L1 capsid proteins. (B) Densitometric measurement of the signals obtained on the film after immunodetection, normalized to the signal for GAPDH.