Table 2.
Selected proteins identified by mass spectrometry in the C. majus latex protein fraction. The table shows the results with score above 200 and arranged according to the decreasing score.
Identified Protein | Accession No. a | Score b | emPAI c |
---|---|---|---|
polyphenol oxidase | m.60893 | 2703 | 4.16 |
reticuline oxidase-like | m.7838 | 2452 | 7.1 |
superoxide dismutase | uniq_01326 | 1764 | 5.62 |
major latex protein (MLP) 28 | m.37901 | 426 | 2.07 |
peroxidase 12-like | m.7868 | 227 | 2.19 |
heat shock 70 kDa mitochondrial-like | m.61661 | 207 | 0.48 |
a Accession numbers of the annotated transcript database of C. majus (version 2 Chmajus2015.01.06) [30] with added cRAP nucleotide sequences (common Random Protein Repository). The searchable Chelidonium majus coding sequence (CDS) database is provided on the IPK Gatersleben ViroBLAST server at: http://webblast.ipk-gatersleben.de/chelidonium/ (accessed on accessed on 24 January 2018). b Mascot search probability-based Mowse score. Ion score is −10 × Log(P), where P is the probability that the observed match is a random event. Score values of individual ions greater than 48 indicate the identity or very high homology of the match between the experimental data and the database sequence (p < 0.05). c Exponentially modified protein abundance index of identified protein according to Mascot search data, emPAI %—proportion of emPAI values in each category to the sum total of emPAI values for each sample (relative abundance). All proteins identified by MS are provided in Table S2.