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. 2022 Aug 19;23(16):9348. doi: 10.3390/ijms23169348

Figure 1.

Figure 1

Characterization of the biological activity of miR-145-5p, miR-101-3p, and miR-335-5p on CFTR gene expression. (A) microRNA binding sites present within the 3′UTR of CFTR mRNA; (B) Venn-diagram indicating the number of mRNA targets of miR-145-5p, miR-101-3p, and miR-335-5p: only CFTR mRNA is a molecular target of all the three microRNAs. Analysis of mRNAs targeted by miRNAs was conducted using TargetScan (release 8.0) and data already in the literature. Afterwards, a Venn-diagram was performed using a Draw Venn Diagram tool (http://bioinformatics.psb.ugent.be/webtools/Venn/, accessed on 9 May 2022); (C) upregulation of CFTR mRNA following treatment of Calu-3 cells with PNAs against miR-145-5p (PNA-a145), miR-101-3p (PNA-a101) and miR-335-5p (PNA-a335). n = 3 independent RT-qPCR experiments (* indicates differences with p < 0.05).