Figure 3.

Effects of combined treatment of Calu-3 cells with PNAs against miR-145-5p and miR-101-3p on CFTR protein accumulation. Calu-3 cells were treated for three days with PNA-a145 and PNA-a101, administered either singularly, or in combination. After these treatments, proteins were isolated and Western blotting was performed. (A) representative results of Western blotting performed using monoclonal antibodies recognizing CFTR or Na⁺/K⁺-ATPase, as indicated; (B) summary of the experiments performed. Results represent the mean fold changes with respect to the control untreated cells of the CFTR/Na⁺/K⁺-ATPase ratios (n = 3 independent quantifications of the same blot; * p < 0.05, ** p < 0.01). The uncut version of the Western blotting gels of (A) and the relative Ponceau staining are included in Supplementary materials (Figure S1). The dotted line represents the sum of the values obtained with single administrations of PNA-a145 and PNA-a101.