FIG. 3.

Gel mobility shift analysis of TRAP complexed with wild-type or 5′ stem-loop deletion trp leader transcripts. 5′-³²P-labeled wild-type (WT) or 5′ stem-loop deletion (Δ5′S-L) trp leader transcripts (0.2 nM) were incubated with 1 mM tryptophan and TRAP at the concentrations indicated above the gel. Each transcript contained the 11 (G/U)AG repeats between +36 and +91. Control reactions without tryptophan were performed with each RNA at the highest TRAP concentration used. Bands corresponding to free RNA (Free) and shifted TRAP-RNA complexes (Bound) are indicated at the left.