FIG. 4.

Deletion of the 5′ stem-loop increases transcription readthrough in vitro. The concentration of TRAP used in each reaction is indicated. Arrows on the left indicate the positions of the 320-nt readthrough transcript (RT) and the 139-nt terminated transcript (T) generated from the wild-type template. Arrows on the right indicate the positions of the 290-nt readthrough and the 109-nt terminated transcripts generated from the 5′ stem-loop deletion template. The molar percentage of readthrough transcripts is shown below each lane.