TABLE 1.
B. subtilis strains used in this study
| Strain | Genotypea | Source or reference |
|---|---|---|
| W168 | Prototroph | BGSCb |
| BG4233 | ΔmtrB argC4 | 20 |
| PLBS44 | amyE::[trpP(−412 to +203)trpE′-′lacZ Cmr] | This study |
| PLBS104 | amyE::[trpP(−412 to +203) Δ(+3 to +32)trpE′-′lacZ Cmr] | This study |
| PLBS251 | ΔmtrB argC4 amyE::[trpP(−412 to +203)trpE′-′lacZ Cmr] | This study |
| PLBS252 | ΔmtrB argC4 amyE::[trpP(−412 to +203) Δ(+3 to +32)trpE′-′lacZ Cmr] | This study |
| PLBS253 | PLBS44/pSI45 (Tcr) | This study |
| PLBS254 | PLBS44/pHY300PLK (Tcr) | This study |
| PLBS255 | PLBS104/pSI45 (Tcr) | This study |
| PLBS256 | PLBS104/pHY300PLK (Tcr) | This study |
| PLBS257 | amyE::[trpP(−412 to +203) C3G trpE′-′lacZ Cmr] | This study |
| PLBS258 | amyE::[trpP(−412 to +203) T5A trpE′-′lacZ Cmr] | This study |
| PLBS259 | amyE::[trpP(−412 to +203) G7A trpE′-′lacZ Cmr] | This study |
| PLBS260 | amyE::[trpP(−412 to +203) C13G trpE′-′lacZ Cmr] | This study |
| PLBS261 | amyE::[trpP(−412 to +203) C15G trpE′-′lacZ Cmr] | This study |
| PLBS262 | amyE::[trpP(−412 to +203) A19T trpE′-′lacZ Cmr] | This study |
| PLBS263 | amyE::[trpP(−412 to +203) G22C trpE′-′lacZ Cmr] | This study |
| PLBS264 | amyE::[trpP(−412 to +203) G24C trpE′-′lacZ Cmr] | This study |
| PLBS265 | amyE::[trpP(−412 to +203) G31C trpE′-′lacZ Cmr] | This study |
| PLBS266 | amyE::[trpP(−412 to +203) C3G G31C trpE′-′lacZ Cmr] | This study |
| PLBS267 | amyE::[trpP(−412 to +203) C15G G22C trpE′-′lacZ Cmr] | This study |
a
Symbols: trpP, the trp promoter; prime, truncation of the gene; −412 to +203, the DNA fragment containing trpP and neighboring regions that was incorporated (numbered relative to the transcription start site); Δ(+3 to +32), the portion of the leader region that was deleted. C3G (and similar designations within genotypes for PLBS257 through PLBS267) indicates the position of a point mutation in the trp leader.
b
BGSC, Bacillus Genetic Stock Center, Ohio State University, Columbus.