Table 1. List of antibodies.
Fluorophore | Target | Species, Isotype | Source | Cat# | Clone | Final dilution |
---|---|---|---|---|---|---|
-- | CD3 | Mouse IgG1 | hybridomas | PTT3 | 1/20 | |
-- | CD8 | Mouse IgG2a | hybridomas | 76-2-11 | 1/4 | |
-- | CD4 | Mouse IgG2b | hybridomas | 74-12-4 | 1/100 | |
-- | CD45 | Mouse IgM | hybridomas | 74-9-3 | 1/2 | |
-- | CD172a | Mouse IgG2b | hybridomas | 74-22-15A | 1/20 | |
PerCP-Cy5.5 | CD4 | Mouse IgG2b | BD | 561474 | 74-12-4 | 1/50 |
FITC | CD14 | Mouse IgG2b | Coulter | 6603511 | 322A-1 My4 | 1/50 |
BV421 | IgG2b | Rat IgG2b | BD | 743174 | II/41 | 1/200 |
PE | IgG2b | Goat IgG2b | BioConcept | 0104–09 | A-1 | 1/100 |
AF488 | IgG1 | Goat IgG | Thermo Fisher | A-21121 | 1/1000 | |
AF647 | IgM | Goat IgG | Thermo Fisher | A-21238 | 1/1000 | |
AF647 | IgG2a | Goat IgG | Thermo Fisher | A-21241 | 1/1000 |