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. 1999 Sep;181(18):5750–5757. doi: 10.1128/jb.181.18.5750-5757.1999

TABLE 1.

Activities of methenyl H4MPT cyclohydrolase, NAD-dependent methylene H4MPT dehydrogenase, and NADP-dependent methylene H4MPT dehydrogenase in cell extracts of different methylotrophic bacteria

Organism Major assimilation pathwaya Grown on: Activity (U/mg)b
Methenyl H4MPT CH Methylene H4MPT DH (NAD) Methylene H4MPT DH (NADP)
α-Proteobacteria:
Methylobacterium extorquens AM1 Serine Methanol 0.7 0.6 5.1
Succinate 0.3 0.5 1.0
 Methylobacterium organophilum XX Serine Methanol 0.3 0.1 1.3
Succinate 0.1 0.1 0.2
 Hyphomicrobium methylovorum GM2 Serine Methanol 0.4 0.1 1.3
 Methylosinus trichosporium OB3b Serine Methane 0.7 0.7 1.0
 Xanthobacter autotrophicus CBB Methanol 0.6 0.1 0.2
Nh + Methanol 0.3 0.03 0.2
 Xanthobacter flavus H4-14 CBB N + Methanol 0.2 0.2 0.4
 Paracoccus denitrificansc CBB Methanol <0.01 <0.01 <0.01
 Rhodopseudomonas acidophilad,e CBB Succinate + Methanol <0.01 <0.01 <0.01
 Rhodobacter sphaeroides 2.4.1f CBB N <0.01 <0.01 <0.01
β-Proteobacteria:
 Methylobacillus flagellatum KT RuMP Methanol 1.0 1.1 1.8
 Methylophilus methylotrophus AS1 RuMP Methanol 0.6 0.2 0.4
γ-Proteobacteria:
 Methylococcus capsulatus Bath RuMP Methane 0.7 0.5 1.7
 Methylococcus thermophilus IIIp RuMP Methane 0.2 0.1 0.3
 Methylomicrobium album BG8 RuMP Methane 0.4 0.3 0.7
 Methylomonas rubra 15sh RuMP Methane 0.6 0.4 0.6
Gram-positive bacteria:
 Bacillus methanolicus MGA3 RuMP Methanol <0.01 <0.01 <0.01
 Amycolatopsis methanolicag RuMP Methanol <0.01 <0.01 <0.01
Glucose <0.01 <0.01 <0.01
a

CBB, Calvin-Benson-Bassham; RuMP, ribulose monophosphate. 

b

Enzyme activities were measured under standard assay conditions at room temperature. CH, cyclohydrolase; DH, dehydrogenase. 

c

Contains a glutathione-linked formaldehyde dehydrogenase (32). 

d

Contains a glutathione-linked formaldehyde dehydrogenase (36). 

e

Cells were grown anaerobically in the light. 

f

Contains a glutathione-linked formaldehyde dehydrogenase (2, 3). 

g

Contains a mycothiol-linked formaldehyde dehydrogenase (27, 28). 

h

N, nutrient agar.