TABLE 1.
Organism | Major assimilation pathwaya | Grown on: | Activity (U/mg)b
|
||
---|---|---|---|---|---|
Methenyl H4MPT CH | Methylene H4MPT DH (NAD) | Methylene H4MPT DH (NADP) | |||
α-Proteobacteria: | |||||
Methylobacterium extorquens AM1 | Serine | Methanol | 0.7 | 0.6 | 5.1 |
Succinate | 0.3 | 0.5 | 1.0 | ||
Methylobacterium organophilum XX | Serine | Methanol | 0.3 | 0.1 | 1.3 |
Succinate | 0.1 | 0.1 | 0.2 | ||
Hyphomicrobium methylovorum GM2 | Serine | Methanol | 0.4 | 0.1 | 1.3 |
Methylosinus trichosporium OB3b | Serine | Methane | 0.7 | 0.7 | 1.0 |
Xanthobacter autotrophicus | CBB | Methanol | 0.6 | 0.1 | 0.2 |
Nh + Methanol | 0.3 | 0.03 | 0.2 | ||
Xanthobacter flavus H4-14 | CBB | N + Methanol | 0.2 | 0.2 | 0.4 |
Paracoccus denitrificansc | CBB | Methanol | <0.01 | <0.01 | <0.01 |
Rhodopseudomonas acidophilad,e | CBB | Succinate + Methanol | <0.01 | <0.01 | <0.01 |
Rhodobacter sphaeroides 2.4.1f | CBB | N | <0.01 | <0.01 | <0.01 |
β-Proteobacteria: | |||||
Methylobacillus flagellatum KT | RuMP | Methanol | 1.0 | 1.1 | 1.8 |
Methylophilus methylotrophus AS1 | RuMP | Methanol | 0.6 | 0.2 | 0.4 |
γ-Proteobacteria: | |||||
Methylococcus capsulatus Bath | RuMP | Methane | 0.7 | 0.5 | 1.7 |
Methylococcus thermophilus IIIp | RuMP | Methane | 0.2 | 0.1 | 0.3 |
Methylomicrobium album BG8 | RuMP | Methane | 0.4 | 0.3 | 0.7 |
Methylomonas rubra 15sh | RuMP | Methane | 0.6 | 0.4 | 0.6 |
Gram-positive bacteria: | |||||
Bacillus methanolicus MGA3 | RuMP | Methanol | <0.01 | <0.01 | <0.01 |
Amycolatopsis methanolicag | RuMP | Methanol | <0.01 | <0.01 | <0.01 |
Glucose | <0.01 | <0.01 | <0.01 |
CBB, Calvin-Benson-Bassham; RuMP, ribulose monophosphate.
Enzyme activities were measured under standard assay conditions at room temperature. CH, cyclohydrolase; DH, dehydrogenase.
Contains a glutathione-linked formaldehyde dehydrogenase (32).
Contains a glutathione-linked formaldehyde dehydrogenase (36).
Cells were grown anaerobically in the light.
N, nutrient agar.