Table 2.
Gene regions and primer pairs used in the current study.
| Gene Region | Sequence (5′-3′) | Product Size (bp) | Optimized PCR Conditions | Reference |
|---|---|---|---|---|
| Translation elongation factor 1 alpha (TEF) | TEF1: ATGGGTAAGGAGGACAAGAC | ≈700 | 95 °C: 5 min, (95 °C: 30 s, 56 °C: 30 s, 72 °C: 1 min) × 35 cycles 72 °C: 10 min | [55] |
| TEF2: GGAAGTACCAGTGATCAT GTT | ||||
| Histone (HIS) | CYLH3F: AGGTCC ACTGGTGGCAAG | ≈500 | 95 °C: 5 min, (95 °C: 30 s, 55 °C: 50 s, 72 °C: 1 min) × 35 cycles 72 °C: 5 min | [56] |
| H3-1b: GCGGGCGAGCTGGATGTCCTT | ||||
| Beta-tubulin (TUB) | BT2a:GGTAACCAAATCGGTGCTGCTTTC | 94 °C: 5 min, (94 °C: 30 s, 54 °C: 50 s, 72 °C: 1 min) × 35 cycles 72 °C: 10 min | [56] | |
| Bt2b: ACCCTCAGTGTAGTGACCCTTGGC | ≈350 |