Table 4.
DNA polymorphism data for F. graminearum isolates based on beta-tubulin (TUB), translation elongation factor 1 alpha (TEF) and histone (HIS) gene sequence comparisons.
Gene Loci | Region of Collection | Number of Isolates (N) | Number of Segregating Sites (S) | Number of Haplotypes (H) | Haplotype Diversity (Hd) | Nucleotide Diversity (π) |
---|---|---|---|---|---|---|
TUB | PB | 47 | 5 | 7 | 0.816 | 0.013 |
HR | 27 | 6 | 10 | 0.900 | 0.019 | |
RJ | 28 | 6 | 11 | 0.865 | 0.015 | |
WB | 21 | 6 | 8 | 0.838 | 0.012 | |
Total | 123 | 6 | 11 | 0.846 | 0.015 | |
TEF | PB | 47 | 197 | 12 | 0.891 | 0.056 |
HR | 27 | 195 | 11 | 0.883 | 0.128 | |
RJ | 28 | 197 | 13 | 0.939 | 0.089 | |
WB | 21 | 196 | 7 | 0.671 | 0.061 | |
Total | 123 | 197 | 15 | 0.888 | 0.080 | |
HIS | PB | 47 | 7 | 14 | 0.920 | 0.006 |
HR | 27 | 7 | 10 | 0.855 | 0.005 | |
RJ | 28 | 7 | 11 | 0.857 | 0.004 | |
WB | 21 | 7 | 10 | 0.919 | 0.006 | |
Total | 123 | 7 | 17 | 0.893 | 0.893 | |
Combined loci | PB | 47 | 384 | 23 | 0.965 | 0.072 |
HR | 27 | 381 | 18 | 0.972 | 0.097 | |
RJ | 28 | 385 | 21 | 0.981 | 0.098 | |
WB | 21 | 381 | 13 | 0.962 | 0.070 | |
Total | 123 | 385 | 30 | 0.974 | 0.083 |
Haplotype diversity and nucleotide diversity are important indicators of population genetic variation. Haplotype and nucleotide diversities are generally considered to be low where haplotype diversity (Hd) and nucleotide diversity (π) are less than 0.5.