TABLE 1.

Agrobacterium strains and plasmids used in this study

Agrobacterium strain or plasmid	Relevant characteristics	Reference or source
Strains
ATvir	GV3101(ppPM6000); pPM6000 is a disarmed Ti plasmid with an intact virulence region	4
ATvirΔD2	Same as ATvir but with a precise nonpolar total deletion of the virD2 gene	6
ATvirΔE2	GV3101(pPM6000E); same as ATvir but with a precise nonpolar deletion of the virE2 gene	31
Plasmids
pTd33	Binary plasmid carrying bacterial resistance to gentamicin; the T-DNA carries the uidA and nptII genes under control of plant regulatory sequences	44
pTd73	Same as pTd33, except that the right border of the T-DNA and the overdrive have been replaced with the OriT sequence of plasmid RSF1010	This work
pMob	pKT231; RSF1010-derived plasmid; carries the genes encoding mobilization proteins MobA, MobB, and MobC; also carries bacterial genes conferring resistance to kanamycin and streptomycin	1