Table 1.
Primers and PCR conditions.
| Target Sequence (Gene) |
Primer Sequence (5′ to 3′) | PCR Conditions | Product Size (bp) |
Source |
|---|---|---|---|---|
| Levansucrase (lsRN) | TGCTCTAGACGATTCCCGCTTATACAGACTATAGAT | 95 °C 3 min, 24× [95 °C 1 min, 55 °C 30 s, 72 °C 4 min] 72 °C 10 min |
1793 | [30] |
| CGGGATCCTTATTTGTTTACCGTTAGTTCTCCC | ||||
| Lichenicidin (bli04127) | GGAAATGATTCTTTCATGG | 95 °C 5 min, 30× [95 °C 1 min, 55 °C 1 min, 72 °C 1 min] 72 °C 5 min |
215 | [31,32] |
| TTAGTTACAGCTTGGCATG | ||||
| Lichenysin synthetase (lchAA) | GTGCCTGATGTAACGAATG | 94 °C 2 min, 30× [94 °C 15 s, 60 °C 30 s, 72 °C 50 s] 72 °C 5 min |
735 | [33] |
| CACTTCCTGCCATATACC |