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. 2022 Aug 5;12(8):1284. doi: 10.3390/jpm12081284

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of radiation on the transmigration (A), invasion (B), and cell survival (C) of MCF-10A cells compared to non-irradiated cells. MCF-10A cells were cultured as mono- or co-culture with fibroblasts; irradiated with 0, 2, or 5 Gy, and treated either with adipose-derived stem cell conditioned medium (ADSC CM) or control CM. Irradiation with 0 Gy was set 1 for each group. (A–C) Columns show the ratio of transmigrated cells (A), invaded cells (B), and the percentage of live cells (C; y-axis) in varying radiation doses and for mono- and co-cultured cells treated with ADSC CM or control CM (x-axis). n = 3 replicate experiments, and values are presented as mean ± SD. *: p < 0.05, and 5 Gy and 2 Gy were compared to 0 Gy (Mann–Whitney U-test).