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Flowchart for laboratory diagnosis of sporotrichosis. KOH: potassium hydroxide; C: carbon source; N: nitrogen source; qPCR: quantitative polymerase chain reaction; RCA: rolling circle amplification; PCR-RFLP: polymerase chain reaction-restriction fragment length polymorphism; ITS: internal transcribed spacer; CAL: calmodulin; BT2: β-tubulin; Ag: antigen detection; Ab: antibody detection; DID: double immunodiffusion; WB: Western blot.
