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. 2022 Jul 31;8(8):809. doi: 10.3390/jof8080809

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Schematic representation of Sporothrix species molecular detection/identification strategies, directly from clinical and/or environmental samples (orange panel) or gDNA extracted from cultured isolates (blue and brown panel). PCR: polymerase chain reaction; PCR-RFLP: polymerase chain reaction-restriction fragment length polymorphism; RCA: rolling circle amplification; qPCR: quantitative polymerase chain reaction; MLSA: multi-locus sequence analysis; AFLP: amplified fragment length polymorphism; SSR: simple sequence repeats; WGS: whole-genome sequencing; RAPD: randomly amplified polymorphic DNA; LoD: limit of detection.