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In Vitro and In Vivo Assessment of Cytotoxicity. (a) Human erythrocytes exposed to P. nigrum extract at 37 °C for 1 h. (b) Cell viability of Vero cells. (c) In vivo toxicity effect of treatment with P. nigrum extract on G. mellonella larvae. The data are expressed as the percentages of survival. Vehicle control: DMSO (<2%). p-values of < 0.05 were used to indicate statistical significance as follows: * p ≤ 0.05. **** p ≤ 0.0001.
