Figure 3.

Neutrophils, monocytes, erythrocytes, and lymphocytes in severe heatstroke. A neutrophil from the animal with severe heatstroke showed a multilobed nucleus (a). Monocytes with platelet aggregates were frequently seen in the samples from the severe heatstroke rats. The red blood cells were stained heterogeneously (a–d). Erythrocytes with blue-red color represent the reticulocyte (polychromatic [polychromatophilic] erythrocyte). The lymphocytes from healthy control rats have a round or oval nucleus and narrow cytoplasm. In contrast, lymphocyte from heatstroke rats has scalloped, angle-edged, or cleaved nuclei with nuclear bodies. The lymphocyte has a relatively large cytoplasm and is namely reactive lymphocyte (c). Other than that, lymphocytes sometimes showed a blast-like change represented by a large, light-stained, dispersed chromatin pattern with prominent nucleoli. These changes were thought to be induced by the damage of heat. Lymphocytes sometimes showed ruptured nuclei (d) or lysed cytoplasmic body that represents oncosis (necrosis).