Figure 7.

Time-lapse changes of leukocytes subjected to heat. Leukocytes were subjected to heat, and sequential changes were observed under the microscope with DAPI. Leukocytes began to shrink at the temperature of 41.5 °C, and some were fragmented, suggesting apoptosis was initiated at 41.5 °C (left). Apoptotic leukocytes started to balloon at 42.0 °C, and at 43.0 °C, most of the leukocytes died in aponecrosis (right). The numbers indicate identical leukocytes in different temperatures. DNA was stained blue with DAPI.