Figure 1.

Phylogenetic assessment, protein structure, domain alignment, and expression of AGC/AKT protein kinase MoSch9 in Magnaporthe oryzae. (A) Maximum likelihood phylogeny constructed with Sch9 proteins homologs across different fungal species including Fusarium graminarium, Beauveria bassiana, Magnaporthe oryzae, Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae, Saccharomyces pombe, and Cryptococcus neoformans. (B) MoSch9 protein structure. MoSch9 is a serine/threonine protein kinase. (C) Sequence alignment showing highly conserved ATP binding and active site in reported fungi. (D) Expression of MoSch9 at different growth phases of M. oryzae. For M, RBM; S, conidiophores stalks formation; Co, conidia; Ge, germination; Ap, appressoria. Vegetative hyphae on 5 day old CM were used as a control stage and assumed as unity (expression level of MoSch9 at hyphal stage on CM = 1). (E) Expression pattern of MoSch9 at different stages of infection development of M. oryzae in rice. Error bars indicate the means ± SD calculated from three independent technical replicates in which triplicate biological samples for each strain were examined in each experiment.