Figure 1.
The domain analysis and multiple amino-acid sequence alignment of TAPL7A and other alginate lyases of PL7 family. (a) The domain of TAPL7A. (b) AlyA5 (CAZ98266.1) from Zobellia galactanivorans DsijT. AlyA (AAA25049.1) from Klebsiella pneumoniae subsp. aerogenes, AlyB (5ZU5) from Vibrio splendidus, PA1167 (AAG04556.1) from Pseudomonas aeruginosa PAO1, AlgAT5 (5ZQI) from Defluviitalea, alyPG (BAA83339.1) from Corynebacterium sp. ALY-1, AlyA1 (CAZ95239.1) from Zobellia galactanivorans DsijT, AlyQ (WP_053404615.1) from Persicobacter sp. CCB-QB2, A1-Ⅱ’ (BAD16656.1) from Sphingomonas sp. A1, FlAlyA (BAP05660.1) from Sphingomonas sp. A1, PsAlg7A (VFY81779.1) from Paradendryphiella salina. Shaded yellow indicates identical and similar amino-acid residues in alginate lyase. The red boxes indicate the positions of the three conserved regions. Black triangles are catalytic bases, red triangles and green triangles represent catalytic acids of PL7 lyase in different red boxes, respectively. Black circles indicate the neutralization residues.
