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Swarming assay for complementation of the flgE mutant SJW1353. Top row, strain SJW1103(wild type [wt]) transformed with the vector pTrc99A. Middle row, SJW1353(flgE) transformed with pTrc99A. Bottom row, SJW1353 transformed with the pTrc99A-based plasmid pKM1500, containing the wild-type flgE gene. Swarms are shown in duplicate and after incubation at 30°C for 5 h.
