Table 3.
Cytotoxic effect of PAC, 5-FU, and their formulations in MCF-7 and MDA-MB-231 cell lines at 24, 48, and 72 h.
| Group | IC50 (µM) | |||||
|---|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | ||||
| MCF-7 | MDA-MB-231 | MCF-7 | MDA-MB-231 | MCF-7 | MDA-MB-231 | |
| Exo * | 15.1 ± 2.2 | 18.3 ± 1.8 | 13 ± 1.6 | 15.8 ± 2.1 | 15.2 ± 1.4 | 17.8 ± 2.2 |
| Free PAC | 0.8 ± 0.2 | 1.1 ± 0.1 | 0.6 ± 0.5 | 0.9 ± 0.2 | 0.78 ± 0.2 | 1.2 ± 0.1 |
| Free 5-FU | 3.8 ± 0.2 | 15.6 ± 1.9 | 2.9 ± 0.5 | 14.6 ± 0.8 | 3.6 ± 0.3 | 14.8 ± 2.2 |
| Free-PAC/5-FU | 0.6 ± 0.3 | 0.9 ± 0.4 | 0.52 ± 0.11 | 0.78 ± 0.1 | 0.69 ± 0.21 | 0.96 ± 0.3 |
| Exo-PAC | 0.63 ± 0.28 | 0.88 ± 0.2 | 0.54 ± 0.1 | 0.76 ± 0.21 | 0.67 ± 0.1 | 0.9 ± 0.22 |
| Exo-5-FU | 3.0 ± 0.21 | 12.2 ± 0.23 | 2.2 ± 0.4 | 10.9 ± 0.54 | 2.9 ± 0.31 | 11.8 ± 0.2 |
| Exo-PAC/5-FU | 0.4 ± 0.2 | 0.6 ± 0.12 | 0.28 ± 0.12 | 0.52 ± 0.3 | 0.48 ± 0.2 | 0.78 ± 0.23 |
| FA-EXO-PAC/5-FU | 0.2 ± 0.1 | 0.50 ± 0.22 | 0.11 ± 0.08 | 0.21 ± 0.22 | 0.18 ± 0.09 | 0.48 ± 0.1 |
* Exosomes concentration were represented in µg/mL and remaining data were represented in moles. Values are represented as mean ± SD (n = 4).