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. 2022 Jul 27;12(8):1130. doi: 10.3390/life12081130

Table 2.

Serial dilution of the cDNA template and corresponding identification number.

Identification Number Dilution Starting Quantity per Reaction (Copies cDNA)
1 Undiluted 105 1
2 1:10 104
3 1:100 103
4 1:1000 102
5 1:10,000 101
6 1:100,000 100

1 One µL of each sample (1–6) was used in the qPCR. Assuming a complete transcription into cDNA, this corresponds to a final concentration of 105 copies/µL cDNA for the undiluted sample.