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Inhibition of the H. volcanii proteasome of α1 and β subunits. Purified proteasome (0.9 μg of protein per ml) was preincubated at 21°C for 60 min with N-acetyl-leucinyl-leucinyl-norleucinal-H (○) or carbobenzoxyl-leucinyl-leucinyl-leucinal-H (●) in 2 M NaCl-Tris buffer with 0.1% (vol/vol) ethanol or 5% (vol/vol) dimethylsulfoxide, respectively. Peptide hydrolysis was then assayed at 60°C with 20 μM Suc-LLVY-Amc at 2.5% (vol/vol) dimethylsulfoxide.
