Skip to main content
. 2022 Jul 27;12(8):1129. doi: 10.3390/life12081129

Figure 2.

Figure 2

DHA reduces radioresistance and mitophagy in A549R cells. (a,b) The survival fractions of A549 and A549R cells, treated with DHA (0–8 μM, 24 h), respectively, were fitted by the multitarget–single hitting model according to a clonogenic assay. (c) Representative fluorescence images of A549 and A549R cells, treated with or without DHA (8 μM, 24 h), and mitochondria and lysosomes of all that were labeled with Mito-Tracker (red) and Lyso-Tracker (green) (×40) (left). Relative fluorescence spot numbers per cell. A total of 100 cells were counted for each analysis (right). (d) Western blot assay of LC3-I, LC3-II, and actin proteins and the relative ratio of LC3-II/I in A549 and A549R cells treated with or without DHA (8 μM, 24 h). (e) The representative images and relative level of ROS in A549 and A549R cells treated with or without DHA (8 μM, 24 h). * p < 0.05 between the indicated groups.