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. 2022 Jul 27;12(8):1129. doi: 10.3390/life12081129

Figure 3.

Figure 3

Inhibition of mitophagy reduces the radioresistance of A549R cells. (a) The survival fractions of A549R cells treated with Mdivi-1 (4 μM, 24 h) and/or DHA (0–8 μM, 24 h) were fitted by the multitarget–single hitting model according to a clonogenic assay. (b) Representative fluorescence images of A549R cells treated with Mdivi-1 (4 μM, 24 h) and/or DHA (8 μM, 24 h) treated with Mito-Tracker (Red) and Lyso-Tracker (Green) (×40) (Left). Relative fluorescence spot numbers per cell. A total of 100 cells were counted for each analysis (right). (c) Western blot assay of LC3-I, LC3-II, and actin proteins and the relative ratio of LC3-II/LC3-I in A549R cells treated with Mdivi-1 (4 μM, 24 h) and/or DHA (8 μM, 24 h). * p < 0.05 between the indicated groups.