Skip to main content
. 2022 Aug 15;18(8):e1010625. doi: 10.1371/journal.ppat.1010625

Fig 3. LAMP1 expression enhances recombinant LCMV/LASV-GPC infection of A549 and DF-1 cells.

Fig 3

(A) Recombinant LCMV/LASV-GPC infection in A549 and DF-1 cells. Cells were allowed to bind LCMV/LASV-GPC in the cold and subsequently incubated at 37°C for 36 h. For infection in A549 and DF-1 cells achieved through forcing viral fusion by low pH exposure, cells were pretreated with 0.2 μM BafA1 for 1 h prior to binding LCMV/LASV-GPC in the cold. Fusion was triggered by applying pH 5.0 citrate buffer at 37°C for 20 min followed by additional incubation in a neutral pH medium at 37°C for 36 h. Infection was detected by immunostaining for LCMV nucleoprotein (NP). (B) Quantification of the fold change of the infection through an endosomal pathway. (C) Quantification of the fold change of the infection through forced fusion. Data shown are means ± SD of three independent experiments. Data were analyzed by Student’s t-test. *, p<0.05; **, p<0.01; ***, p<0.001.